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中国精品科技期刊2020
尤玲玲, 陈永慧, 刘金福, 靳晓明, 冯超. 苦瓜皂苷对胰岛素抵抗HepG2细胞葡萄糖消耗量的影响[J]. 食品工业科技, 2014, (05): 338-340. DOI: 10.13386/j.issn1002-0306.2014.05.062
引用本文: 尤玲玲, 陈永慧, 刘金福, 靳晓明, 冯超. 苦瓜皂苷对胰岛素抵抗HepG2细胞葡萄糖消耗量的影响[J]. 食品工业科技, 2014, (05): 338-340. DOI: 10.13386/j.issn1002-0306.2014.05.062
YOU Ling-ling, CHEN Yong-hui, LIU Jin-fu, JIN Xiao-ming, FENG Chao. Influence of bitter melon saponin on the glucose consumption of insulin resistance HepG2 cell in vitro[J]. Science and Technology of Food Industry, 2014, (05): 338-340. DOI: 10.13386/j.issn1002-0306.2014.05.062
Citation: YOU Ling-ling, CHEN Yong-hui, LIU Jin-fu, JIN Xiao-ming, FENG Chao. Influence of bitter melon saponin on the glucose consumption of insulin resistance HepG2 cell in vitro[J]. Science and Technology of Food Industry, 2014, (05): 338-340. DOI: 10.13386/j.issn1002-0306.2014.05.062

苦瓜皂苷对胰岛素抵抗HepG2细胞葡萄糖消耗量的影响

Influence of bitter melon saponin on the glucose consumption of insulin resistance HepG2 cell in vitro

  • 摘要: 采用高浓度的胰岛素诱导HepG2细胞出现葡萄糖代谢异常,从胰岛素的添加量和作用时间角度探讨建立胰岛素抵抗细胞(HepG2/IR)模型的最佳条件;分别设立阴性对照组、胰岛素抵抗模型组、盐酸吡格列酮阳性对照组以及苦瓜皂苷干预组(100、300、500、700μg/mL),利用葡萄糖测定试剂盒检测各组葡萄糖消耗量,同时MTT法评价苦瓜皂苷对HepG2/IR细胞活性的影响。结果表明,30μg/mL胰岛素诱导处理HepG2细胞36h是产生胰岛素抵抗的最适条件。与HepG2/IR模型组相比,300μg/mL的苦瓜皂苷不仅可以显著改善HepG2/IR细胞的葡萄糖消耗量,还能提高其细胞活性。 

     

    Abstract: High concentration insulin was added into HepG2 cells to induce glucose metabolism abnormal.The optimal conditions of insulin resistant HepG2 cells ( HepG2 /IR) model was investigated from insulin dose and effect time.HepG2 cells were divided into control group, insulin resistant group, pioglitazone hydrochloride group, bitter melon saponin groups ( 100, 300, 500, 700μg /mL) . The glucose consumption was measured by glucose oxidase method ( GOD) .The cell activity was estimated by MTT method.These results showed that treatment with 30μg /mL insulin for 36h was the best condition for induction of HepG2 /IR model.Compared with HepG2 /IR group, treatment with 300μg /mL bitter melon saponin not only could alleviate insulin resistant HepG2 cells significantly, but cell activity was increased.

     

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