Abstract: The determination method of ethyl carbamate based on acetylcholinesterase inhibion was researched in this paper. It was found that inhibition rate of acetylcholinesterase activity by ethyl carbamate was significantly improved after the sensibilization by bromine solution. There was a linear relationship between inhibition rate of acetylcholinesterase activity and concentration of bromine solution in a certain range.The established determination method of ethyl carbamate had good linearity in a range of 1⁷.5mg /L and linear correlation reached 0.9972 and detection limit reached 0.04177mg /L.RSD was between 1.82% to 9.69%.The method which is easy to operate and control because of rapid, simple, and required less equipment under mild conditions, is low cost. This paper provide certain theoretical and technical support for further development of rapid detection technologies and products of ethyl carbamate based on enzyme inhibition in products of alcoholic beverages and fermented foods