• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
张智, 于震, 王振宇, 邓心蕊, 刘冉, 张巧俏. 落叶松树皮多酚纯化工艺研究[J]. 食品工业科技, 2014, (05): 187-191. DOI: 10.13386/j.issn1002-0306.2014.05.029
引用本文: 张智, 于震, 王振宇, 邓心蕊, 刘冉, 张巧俏. 落叶松树皮多酚纯化工艺研究[J]. 食品工业科技, 2014, (05): 187-191. DOI: 10.13386/j.issn1002-0306.2014.05.029
ZHANG Zhi, YU Zhen, WANG Zhen-yu, DENG Xin-rui, LIU Ran, ZHANG Qiao-qiao. Study on purification technology of polyphenols from larch barks[J]. Science and Technology of Food Industry, 2014, (05): 187-191. DOI: 10.13386/j.issn1002-0306.2014.05.029
Citation: ZHANG Zhi, YU Zhen, WANG Zhen-yu, DENG Xin-rui, LIU Ran, ZHANG Qiao-qiao. Study on purification technology of polyphenols from larch barks[J]. Science and Technology of Food Industry, 2014, (05): 187-191. DOI: 10.13386/j.issn1002-0306.2014.05.029

落叶松树皮多酚纯化工艺研究

Study on purification technology of polyphenols from larch barks

  • 摘要: 本文采用大孔树脂分离技术对落叶松树皮多酚进行纯化。通过吸附与解析实验筛选出适合进行纯化工艺的大孔树脂。以多酚回收率和纯度为响应值,分别考察径长比、乙醇浓度、上样浓度、洗脱流速、进样体积和pH六个因素对多酚纯化效果的影响。利用Design-expert8.05软件设计3因素3水平响应面优化实验并处理数据,结合实际操作需要,确定了多酚纯化的最佳工艺条件为径长比1∶25、洗脱浓度47%、上样浓度2.5mg/mL、洗脱流速5mL/min、进样体积为42mL、pH6.85,在此条件下多酚纯度的验证值为75.66%,验证实测值是75.52%。通过高效液相色谱仪分析该纯化液,发现落叶松树皮有5个单体酚,分别是没食子酸、儿茶素、绿原酸、咖啡酸与芦丁,其中儿茶素的含量最高。 

     

    Abstract: Macroporous resin separation was established to purify the polyphenols from larch barks.Adsorption and desorption experiments were carried out to select the suitable macroporous resin for purification process. As the response to recovery and purity of polyphenols, 6 factors such as fineness ratio, elution concentration, sample concentration, eluting speed, sample volume, and pH, were used to evaluate purification effect of polyphenols.Response surface experiment was established about three independent variables with three levels by the tool of Design-expert8.05 and the data was manipulated with it. Combined with actual needs, the optimal technological purification conditions of polyphenols were as follows: fineness ratio 1 ∶ 25, elution concentration 47%, sample concentration 2.5mg /mL, eluting speed 5mL /min, sample volume 42mL and pH6.85, in this process the verification value of purity of polyphenols was 75.66% and measured value of that was 75.52%. Analyzed through high performance liquid chromatograph, the purified liquid was discovered to contain 5 polyphenol monomers including gallic acid, catechin, chlorogenic acid, caffeic acid and ruitin, among of which the highest content of catechin was analyzed.

     

/

返回文章
返回