Abstract: Pecinase and cellulase were immobilized on sodium alginate and was applied in the extraction of lycopene from tomato paste. Based on single factor experiments, response surface optimization method was used to determine the optimal conditions for immobilization. Results showed that the using immobilized pectinase and cellulase could improve the extraction rate of lycopene from tomato paste and the best time, pH, extraction temperature, enzyme-tomato mass ratio value were 2.8h, 4.0, 50℃ and 1.00g/100g respectively. Under these conditions, the extraction rate of lycopene was 3.68μg/mL. The immobilized pectinase and cellulase showed high stability, and could be used for 7 batches and its properties still kept fine performance.