Abstract: The influence of ethanol, oxalic acid, tartaric acid, pyruvic acid, malic acid, lactic acid, acetic acid, citric acid, succinic acid on Acetobacter xylinum producing bacterial cellulose were studied. These results showed that:pyruvic acid, lactic acid, acetic acid, ethanol, malic acid, citric acid and succinic acid had stimulative effect on Acetobacter xylinum producing bacterial cellulose. Oxalic acid and tartaric acid had inhibitory effect on Acetobacter xylinum producing bacterial cellulose. The optimum additive concentration of ethanol and organic acids were as follows:pyruvic acid 0.15%, malic acid 0.1%, lactic acid 0.3%, acetic acid 0.4%, citric acid 0.1%, succinic acid 0.2%, ethanol 4%. By the orthogonal experiment the optimum proportion of mixed acid were as follows:0.1% pyruvic acid, malic acid 0.12%, 0.2% lactic acid, acetic acid 0.3%, citric acid 0.12%, succinic acid 0.2%, 3% ethanol. The BC production was 3.012g/L, which was 2.03 times of blank group.