• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
夏姣, 蒲彪, 敖晓琳, 陈安均, 崔慧玲, 袁杨. 传统四川泡菜发酵过程中明串珠菌的分离鉴定[J]. 食品工业科技, 2014, (04): 153-156. DOI: 10.13386/j.issn1002-0306.2014.04.058
引用本文: 夏姣, 蒲彪, 敖晓琳, 陈安均, 崔慧玲, 袁杨. 传统四川泡菜发酵过程中明串珠菌的分离鉴定[J]. 食品工业科技, 2014, (04): 153-156. DOI: 10.13386/j.issn1002-0306.2014.04.058
XIA Jiao, PU Biao, AO Xiao-lin, CHEN An-jun, CUI Hui-ling, YUAN Yang. Separation and identification of Leuconostoc during the fermentation of sichuan pickles[J]. Science and Technology of Food Industry, 2014, (04): 153-156. DOI: 10.13386/j.issn1002-0306.2014.04.058
Citation: XIA Jiao, PU Biao, AO Xiao-lin, CHEN An-jun, CUI Hui-ling, YUAN Yang. Separation and identification of Leuconostoc during the fermentation of sichuan pickles[J]. Science and Technology of Food Industry, 2014, (04): 153-156. DOI: 10.13386/j.issn1002-0306.2014.04.058

传统四川泡菜发酵过程中明串珠菌的分离鉴定

Separation and identification of Leuconostoc during the fermentation of sichuan pickles

  • 摘要: 分别以红皮萝卜、卷心菜、豇豆为原料,用自然发酵法制作了三种四川泡菜。对三种泡菜发酵过程中的明串珠菌进行分离,主要得到四种不同形态的菌株,将其分别命名为:MC-1、MC-2、MC-13、J-5,其中J-5为豇豆泡菜特有,其余为三种泡菜共有。采用生理生化结合16SrDNA序列鉴定对分离的菌株进行鉴定,依次鉴定为:乳明串珠菌(L.lactis)、肠膜明串株菌葡聚糖亚种(L.mesenteroides subsp.dextranicum)、柠檬明串珠菌(L.citreum)、假肠膜明串珠菌(L.pseudomesenteroide)。对发酵过程中明串珠菌动态分析表明:L.lactis、L.mesenteroides subsp.dextranicum为三种泡菜中的主要优势明串珠菌;L.citreum在发酵第24d出现后很快又消失;L.pseudomesenteroide在豇豆发酵的第39d比较活跃;泡菜发酵到第11d,泡菜水中所有的L.lactis都消失。 

     

    Abstract: The Leuconostoc throughout spontaneous fermentation of three kinds of Sichuan pickles were analyzed. Four strains of different colony morphology were separated, and named MC-1, MC-2, MC-13, J-5. J-5 only existed in the bean pickle, others existed in all kinds of pickles. After some physiological and biochemical tests and sequencing the 16SrDNA sequence of the four strains, MC-1 was identified as L.lactis, and MC-2 was L.mesenteroides subsp.dextranicum, MC-13 was L.citreum and J-5 was L.pseudomesenteroide. Dynamic results showed that L.lactis and L.mesenteroides subsp.dextranicum were the dominant Leuconostoc, they presented in the brine as soon as vegetables pickled, L. citreum presented in the 2 ~ 4th day and disappeared soon, L.pseudomesenteroide existed in 39th day. All the L.lactis disappeared till the 11th day.

     

/

返回文章
返回