Abstract: In order to explore the reliable method of whole milk powder origin traceability, 6 kinds of whole milk powder came from different regions were regarded as object of study. The physicochemical analysis was used to measure samples ' content of protein, fat, ash and solubility, at the same time Fourier transform infrared spectrometer was used to obtain all samples' FTIR spectra. Then SPSS was used to analyze the data by analysis of variance, similarity analysis and cluster analysis. Results showed that the content of protein and fat of whole milk powder came from different regions had significant difference, and ash content and solubility were relatively stable. FTIR macroscopically fingerprint had a certain similarity, but the peak position and peak shape of the characteristic peaks had difference, and to a certain extent, reflect the geographical environment influence on the physicochemical properties of milk powder. SPSS statistical software was used to process physicochemical properties and FTIR results, the producing area identification of 6 kinds of milk powder was realized, the accuracy of cluster analysis achieved 100%.