Abstract: In recent years, the bioactive peptide derived from food was more increasingly applied to functional foods, leading to the research on active peptide become a hot study topic on the food field. Preparation methods of bioactive peptide derived from food, containing enzymatic preparation, microbial fermentation and synthetic preparation, were introduced briefly, according to molecular weigher and electric charge of food-borne bioactive peptide. It was focused on the separation and purification of bioactive peptide derived from food.