树舌富锗深层培养研究

Study on the ge-accumulation of Ganoderma applanatum in submerged culture

摘要: 研究了不同浓度的锗对树舌液体培养生物量、菌丝体中有机锗、胞内多糖、腺苷及胞外多糖等主要活性成分含量的影响。结果表明,锗浓度在50~250μg/mL的范围内,对树舌菌丝体主要活性成分合成有很好促进作用,在锗浓度为250μg/mL时,生物量、有机锗、胞内多糖、腺苷均达最高,分别为15.44g/L、588μg/g、24.88mg/g、1.41mg/g,分别比对照组提高了33.1%、1267.4%、73.9%、101.4%。胞外多糖含量在锗浓度为150μg/mL时达到1.12g/L,比对照组提高了31.7%。

Abstract: The effects of different concentrations of inorganic germanium on biomass, the contents of organic germanium, extracellular polysaccharide, adenosine and intracellular polysaccharide in submerged culture of Ganoderma applanatum were studied. Results showed that 50 ~ 250μg /mL of germanium in the culture promoted the content of main active components in the mycelia and fermentation broth.When the level of germanium was 250μg /mL, the content of biomass, intracellular polysaccharide and adenosine reached the peak, 15.44g /L, 588μg /g, 24.88mg /g, 1.41mg / g, respectively.As compared with the control bank, these increased by 33.1%, 1267.4%, 73.9%, 101.4%.The extracellular polysaccharide reached 1.12g /L and increased 31.7% compared with the control bank when the level of germanium was 150μg /mL.