Abstract: Naringinase was immobilized into alginate sodium matrices with glutaraldehyde via crosslinked- entrapment- crosslinked technique. According to the orthogonal test design based on the single factor experiments, the optimal immobilization conditions were obtained as follows: the mass concentration of alginate sodium 3.0%, the amount of enzyme onto supports 0.01mg /g, the previous crosslinking volume fraction of glutaraldehyde 2.0%, the previous crosslinking time 1.5h, the after crosslinking volume fraction of glutaraldehyde 0.025%, the after crosslinking time 2h, and the highest activity of immobilized naringinase was 5.07U /g.Meanwhile, operational properties of the immobilized naringinase were investigated. The results showed that the temperature tolerance and storage stability of immobilized naringinase had dramatically increased than the free enzyme. The immobilized naringinase retained about 60% of initial activities after 7 recycles ( 60℃, pH4.0) .