Abstract: Using crude propolis of Mengzi Yunnan as raw materials, different propolis extraction were acquired in 75% ethanol, 95% ethanol, 75% ethyl acetate and 95% ethyl acetate at room temperature.The flavonoids contents of propolis extract were determinated, and the inhibition of propolis extract on the Volvariella volvacea and contaminative microorganisms which isolated from contaminative V.volvacea strains was discussed.The results showed that the most soluble content was 60.3% and most flavonoids content was 6.23mg/mL, under the conditions of 95% ethyl acetate extraction.After preliminary identification, contaminative microorganisms were gram-positive bacteria and Penicillium.Propolis extract had inhibitory effect on both P.eryngii and contaminative microorganisms, the inhibition degree was bacteria>V.volvacea>Penicillium.Correlation analysis indicated that all of the correlation coefficients on the inhibitory rate of bacteria, V.volvacea and Penicillium with the flavonoids content were above 0.9, which showed a good correlation.