壳聚糖-PE双层抑菌保鲜膜的制备及抑菌性的研究

Preparation of chitosan-PE antimicrobial bi-layer films and antibacterial activity

摘要: 结合壳聚糖良好的成膜性和广谱抑菌性及PE膜较好的机械强度和阻隔性,制备壳聚糖-PE双层抑菌保鲜膜。通过单因素实验和Box-Behnken Design实验设计考察壳聚糖、醋酸、甘油和Span 40浓度对膜的抗拉强度和断裂伸长率的影响;得到膜液最佳组合为:壳聚糖浓度0.94%、醋酸浓度0.49%、甘油浓度1.07%、Span 40浓度1.41%,所得膜的抗拉强度和断裂伸长率分别为3.59MPa和116.72%;最后研究了壳聚糖-PE双层抑菌保鲜膜对大肠杆菌（E.Coli）和金黄色葡萄球菌（B.Subtilis）的抑菌性,结果表明,此保鲜膜对大肠杆菌和金黄色葡萄球菌的抑菌率分别可达80.77%和93.02%。

Abstract: Chitosan-PE films were prepared by combining the excellent film property and antimicrobial activity of chitosan with good mechanical and barrier properties of PE film. The effect of the concentration of chitosan, acetic acid, glycerol (Gly) and Span 40 on tensile strength (TS) and elongation at break (E) were investigated.The optimum combine ingredients were obtained through single factor experiments and Box-Behnken Design, which were 0.94% chitosan, 0.49% acetic acid, 1.07% Gly and 1.41% Span 40. TS and E of chitosan-PE bi-layer films were 3.59MPa and 116.72%, respectively. Also, the antibacterial activity against E. Coli and B. Subtilis of the film were measured. Results showed that the antibacterial ratio was 80.77% and 93.02%, respectivedly.