Abstract: Breast and thigh muscle of rejected layer was used as the raw material. Study on the compound tenderizing technology of rejected layer's muscle was based on response indicators, the reduced rate of shear force and water holding capacity, by papain dosage, vacuum tumbling time, tenderized static temperature and time. Optimal technological parameters were obtained as follow:papain dosage 3.0U/g, vacuum tumbling time28min, tenderized temperature 20℃, tenderized static time 40min, the reduced rate of WBS of the breast muscle was 50.9%, water holding capacity 92.7%. Papain dosage 2.76U/g, vacuum tumbling time 30min, tenderized temperature 23℃, tenderized static time 38min, the reduced rate of WBS (Warner-Bratzler Shear) of the thigh muscle was 52.2%, water holding capacity 87.2%. Results showed that the compound tenderizing technology, combining mechanical rolling and enzymolysis, could effectively improve the tenderness and water holding capacity of rejected layer's muscle.