四角蛤蜊废弃肉渣制备ACE抑制肽的工艺研究

Study on optimization of preparation of ACE-inhibitory peptides from Mactra veneriformis flesh residue

摘要: 采用胰蛋白酶水解四角蛤蜊废弃肉渣制备ACE抑制肽。以体外ACE抑制率为指标,在单因素实验的基础上,通过响应面法优化酶解工艺。结果表明,胰蛋白酶水解四角蛤蜊肉渣制备ACE抑制肽的最佳工艺条件为:酶解温度48℃,pH为8.50,胰蛋白酶加入量为0.60%,酶解时间为2h,酶解产物的平均ACE抑制率为77.08%,与预测值77.22%的相对误差为0.18%,证明响应面法对于酶解肽工艺优化具有指导意义。

Abstract: Trypsin was used to prepare ACE inhibitory peptides from flesh residue of Mactra veneriformis. Based on the single factor experiments, response surface method was used to optimize the hydrolysis conditions. The best conditions were as follows:hydrolysis temperature 48℃, hydrolysis pH8.50, enzyme dosage 0.60%, hydrolysis time 2 hours. The ACE inhibition ratio of hydrolysis peptides at the best conditions was 77.08%, compared with the theoretical value of 77.22%, the relative error was 0.18%. It suggested that the response surface method made sense on hydrolysis conditions optimization.