响应曲面法优化碱水解谷维素制取阿魏酸的工艺

Optimization of the preparing processing of ferulic acid from γ-oryzanol with alkaline hydrolysis using response surface methodology

摘要: 以谷维素为原材料,利用响应面法优化其碱水解制取阿魏酸的工艺。在单因素实验的基础上,以水解时间、碱浓度、底物浓度为影响因素,应用Box-Behnken中心组合方法进行三因素三水平实验,以阿魏酸得率为响应值,进行响应面分析。结果表明,碱水解谷维素制取阿魏酸的最佳工艺条件为:水解时间12.5h,碱浓度（NaOH质量分数）为12%,底物浓度6.57mg/mL,阿魏酸得率的理论预测值为72.68%,验证值为71.33%;与预测值相对误差仅为1.89%。此工艺利用谷维素用碱水解制取阿魏酸,其方法可靠、易行,得率较高。

Abstract: Response surface methodology (RSM ) was used to optimize the processing of alkaline hydrolysis for γ-oryzanol to improve the preparing yield of ferulic acid. On the basis of single factor experiments,a three-factor,three-level Box-Behnken central composite design(CCD) was utilized for the setting of mathematical model and the predicting of ferulic acid preparing yield. The optimum conditions for the preparation of ferulic acid were as follows:hydrolysis time 12.5h,concentration of NaOH 12%,substrate concentration 6.57mg/mL. Under such conditions,the model -predicted and experimental values of ferulic acid yield were 72.68% and 71.33% respectively,revealing 1.89% relative error between them. The developed preparing process was simple, reliable and highly efficient.