发酵玉米黄秸秆乳酸菌的分离纯化及发酵条件的优化

Purification lactic acid bacteria from fermented corn yellow straw and optimization of fermentation conditions

摘要: 从17种天然发酵的玉米秸秆饲料中得到LB2、LC2两株乳酸菌,对所分离的菌株进行形态特征、生理生化特性和16SrDNA序列分析,并得到一株产酸量较高的乳酸菌LC2,将其作为实验菌种。对影响秸秆发酵的因素进行了研究,影响秸秆发酵的主要因素是接种量、发酵温度及发酵时间。结果表明,较适宜的发酵条件为发酵温度30℃,接种量1%,发酵时间为10d。玉米黄秸秆经发酵,其中蛋白质含量达到14.35%。

Abstract: Two Lactobacillus LB2 and LC2, were screened from seventeen nature fermented maize straws. Based on the results of their morphological, physiological, biochemical properties, as well as 16S rDNA sequence analysis, LB2 and LC2 were preliminarily identified as Lactobacillus LC2 was selected as an experimental bacterium according to its high lactic acid output. The factors affecting fermented maize straw had been studied. The optimum fermentation conditions were as following:the suitable inoculating amount of Lactobacillus LC2 1%, temperature 30℃, and time 10d. The protein content in fermented maize straw increased even up to 14.35%.