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中国精品科技期刊2020
海水中溶藻弧菌的分离、鉴定及其耐药性分析[J]. 食品工业科技, 2013, (16): 195-199. DOI: 10.13386/j.issn1002-0306.2013.16.009
引用本文: 海水中溶藻弧菌的分离、鉴定及其耐药性分析[J]. 食品工业科技, 2013, (16): 195-199. DOI: 10.13386/j.issn1002-0306.2013.16.009
Isolation,identification and antimicrobial resistance research of Vibrio alginolyticus isolated from seawater[J]. Science and Technology of Food Industry, 2013, (16): 195-199. DOI: 10.13386/j.issn1002-0306.2013.16.009
Citation: Isolation,identification and antimicrobial resistance research of Vibrio alginolyticus isolated from seawater[J]. Science and Technology of Food Industry, 2013, (16): 195-199. DOI: 10.13386/j.issn1002-0306.2013.16.009

海水中溶藻弧菌的分离、鉴定及其耐药性分析

Isolation,identification and antimicrobial resistance research of Vibrio alginolyticus isolated from seawater

  • 摘要: 用无菌采样器在上海临港海域采集100份海水,经过增菌培养和选择性分离后,进行3种生理生化鉴定和PCR鉴定,共得到33株溶藻弧菌。采用优化的K-B法测定分离菌株对20种抗生素的耐药性,结果表明:溶藻弧菌对四环素的耐药性最高(100.0%),其次是氨苄青霉素(97.0%)和羧苄青霉素(93.9%);对头孢吡肟、安曲南、红霉素、环丙沙星、诺氟沙星和氯霉素敏感。本次分离的溶藻弧菌具有严重的多重耐药性,所有分离菌均对3种及3种以上抗生素多重耐药,同时对6种及6种以上抗生素耐药的有15株(45.5%),同时对8种及8种以上抗生素耐药的有6株(18.2%)。本次关于溶藻弧菌对抗生素的耐药性分析结果可以为食源性疾病控制提供参考依据,同时为溶藻弧菌的耐药机制的研究奠定理论基础。 

     

    Abstract: A total of 100 seawater samples were collected by sterile sampler at Lingang sea area of shanghai, and 33 Vibrio alginolyticus isolates were obtained after cultured,separated and identified by physiological biochemical test and PCR rapid detection method.All isolates were tested for susceptibility to 20 kinds of antibiotics by improved Kirby -Bauer method. Susceptibility test showed that all strains were resistant to tetracycline(100.0%),followed by ampicillin(97.0%) and carbenicillin(93.9%). However,they were sensitive to cefepime,aztreonam,erythromycin,ciproffloxacin,norfloxacin and chloramphenicol. All isolates showed multiple resistance to no less than 3 antibiotics ,15 (45.5% ) isolates showed multiple resistance to no less than 6 antibiotics,and 6(18.2%) isolates showed multiple resistance to no less than 8 antibiotics,indicating that all the V. alginolyticus which we isolated have strong multiple resistance to many antibiotics. This study can provide reference for controlling the occurrence of foodborne diseases,and establish the theoretical base to illustrate the antibiotic resistance mechanism of Vibrio alginolyticus.

     

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