山桃稠李果实花色苷对HepG2细胞抗氧化系统的影响

Effect of anthocyanins extracted from Prunus maackii on the antioxidant function of HepG2 cells

摘要: 目的:研究山桃稠李果实花色苷对人肝癌细胞（HepG2）抗氧化系统的影响。方法:质量浓度0.1～1.2mg/mL的花色苷作用于HepG2细胞,MTT法检测HepG2细胞生长抑制率;检测各组细胞内抗氧化酶活性和谷胱甘肽、丙二醛的含量变化;采用DCFH-DA探针检测细胞内活性氧（ROS）含量的变化。结果:随着花色苷质量浓度的增加,HepG2细胞的抗氧化酶（T-SOD、GSH-PX）活性显著降低;谷胱甘肽（GSH）含量下降,丙二醛（MDA）及活性氧的含量上升。结论:山桃稠李果实花色苷能够明显的降低HepG2细胞的抗氧化能力。

Abstract: Objective: To study the anthocyanins extracted from Prunus maackii on the antioxidant function of HepG2 cells.Methods: The anthocyanins of different mass concentration extracted from Prunus maackii effect on HepG2 cells, the inhibition rate of anthocyanins on the proliferation of cell was tested by the method of MTT.The activity of antioxidant enzymes and the content of Glutathione and MDA were tested.The content of ROS by DCFH-DA probe was tested. Results: As the mass concentration of anthocyanins increased, the activity of antioxidant enzymes decreased obviously, the content of glutathione decreased and the content of MDA and reactive oxygen species increased.Conclusion: the anthocyanins extracted from Prunus maackii could significantly impair the antioxidant ability of HepG2 cells.