离子交换树脂对马尾松松针中莽草酸的分离纯化

Separation and purification of shikimic acid from pine needles with ion exchange resin

摘要: 比较4种吸附树脂对马尾松松针提取液中莽草酸的静态吸附和解吸效果,从中筛选出适合该提取液中莽草酸分离纯化的树脂,并对其动态吸附-解吸性能进行研究。结果表明,331阴离子交换树脂最适合马尾松松针提取液中莽草酸的纯化。当上样液的质量浓度为1.5mg/mL,上样流速为2mL/min,上样液体积为4BV时,331阴离子交换树脂达到动态吸附饱和。再用8BV2%的氢氧化钠溶液,以4mL/min的流速可以完全洗脱。经过331阴离子交换树脂的纯化,莽草酸的纯度由原来的25%提高到90%,纯化提取率为90.9%。

Abstract: The adsorption and desorption rates of 4 types of adsorption resins towards shikimic acid from the Pine Needles were investigated.Among the studied ion exchange resin, 331 anion exchange resin was the most suitable to purify crude shikimic acid from the Pine Needles.When 4BV of 1.5mg /mL sample was flowed through 331 anion exchange resin column at a speed of 2mL /min, the adsorption of the 331 anion exchange resin reached dynamic equilibrium.The adsorbed shikimic acid could be completely eluted with 8BV of 2% sodium hydroxide solution at 4mL / min.After the purification, the purity of shikimic acid was increased from 25% to 90% , resulting in a purification yield of 90.9%.