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中国精品科技期刊2020
弱后酸化保加利亚乳杆菌突变株与亲本菌株H+-ATPase基因的相似性比较[J]. 食品工业科技, 2013, (15): 127-130. DOI: 10.13386/j.issn1002-0306.2013.15.001
引用本文: 弱后酸化保加利亚乳杆菌突变株与亲本菌株H+-ATPase基因的相似性比较[J]. 食品工业科技, 2013, (15): 127-130. DOI: 10.13386/j.issn1002-0306.2013.15.001
Comparison of similarity of H+-ATPase coding gene between wild strain and mutant strain with low post-acidification of Lactobacillus delbrueckii subsp.bulgaricus[J]. Science and Technology of Food Industry, 2013, (15): 127-130. DOI: 10.13386/j.issn1002-0306.2013.15.001
Citation: Comparison of similarity of H+-ATPase coding gene between wild strain and mutant strain with low post-acidification of Lactobacillus delbrueckii subsp.bulgaricus[J]. Science and Technology of Food Industry, 2013, (15): 127-130. DOI: 10.13386/j.issn1002-0306.2013.15.001

弱后酸化保加利亚乳杆菌突变株与亲本菌株H+-ATPase基因的相似性比较

Comparison of similarity of H+-ATPase coding gene between wild strain and mutant strain with low post-acidification of Lactobacillus delbrueckii subsp.bulgaricus

  • 摘要: 为了确定德氏乳杆菌保加利亚亚种H+-ATPase的调控机制,以H+-ATPase缺陷的德氏乳杆菌保加利亚亚种突变菌株和亲本菌株KLDS1.9201作为研究对象。首先分别提取亲本和突变菌株的基因组DNA,随后利用PCR的方法扩增出H+-ATPase蛋白的全部编码基因并测序,最后利用DNAMAN软件比较亲本和突变菌株的H+-ATPase的相似性。结果表明亲本和突变菌株H+-ATPase编码基因的相似性为100%。H+-ATPase缺陷的德氏乳杆菌保加利亚亚种突变菌株KLDS1.9201-11和亲本菌株KLDS1.9201的H+-ATPase活力之间的差异并不是由于编码基因发生了突变,可能是转录水平的降低导致H+-ATPase酶蛋白的表达量降低,进而影响酶活力。为进一步研究德氏乳杆菌保加利亚亚种H+-ATPase的调控机制奠定了基础。 

     

    Abstract: In order to determine the regulation mechanism of H + - ATPase of Lactobacillus delbrueckii subsp. bulgaricus, a H + - ATPase deficiency strain Lactobacillus delbrueckii subsp. bulgaricus KLDS1.9201 - 11 with low post - acidification which was derived from Lactobacillus delbrueckii subsp. bulgaricus KLDS1.9201 in previous research and the wild type strain KLDS1.9201 were investigated.The genomic DNA was extracted from KLDS1.9201 -11 and KLDS1.9201 respectively, and the coding genes of H+-ATPase of these two strains were acquired by PCR amplification, then PCR products were sequenced.At last the similarity of coding genes of H+-ATPase between the KLDS1.9201-11 and KLDS1.9201 was compared by the software of DNAMAN.Results showed that the similarity of coding genes of H+-ATPase between the KLDS1.9201-11 and KLDS1.9201 was 100%.In conclusion the differential of H+-ATPase activity of KLDS1.9201-11 and KLDS1.9201 was not caused by the mutation of coding gene of H + - ATPase.And it might the lower expression of H+-ATPase of mutant strain KLDS1.9201-11 which in turn caused the reduced H+-ATPase activity.It provided the theoretical basis for further investigating the regulation mechanism of H+-ATPase of Lactobacillus delbrueckii subsp.bulgaricus.

     

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