Abstract: Microecological products are paid more and more attention.Most commercial microecological products on local market are the yogurts which contain lactic acid bacteria.The common technology of strain identification are the physiological and biochemical test or the application of PCR with some defects such as time-consuming or need specialized person.We used Riboprinter to complete the subspecies level of identification of 16 lactobacillus bacterias isolated from 11 yogurt samples based on the separation and purification of the bacterias.Not only chose the optimization of the culture conditions, but also established the “Traceability File” used for the quality research.What’s more, the use of the 16s rDNA fingerprint analysis and real time PCR helped to confirm the verification of the results from the colony identification.