Abstract: In order to study the extraction technology of Antarctic Krill polysaccharide, the hot low-concentration alkali solution extraction combining with enzymolysis technology methods were used.The influence of liquid ratio, extraction temperature, extraction time on the yield of Antarctic Krill polysaccharide were studied.On the basis of the single-factor experiments, the results of response surface methodology showed that the optimum extraction conditions were liquid ratio of 1∶2.5 (g/mL) , extraction temperature of 92℃, extraction time of 3.5h, and the yield of Antarctic Krill polysaccharide was 0.288% .The result showed that establishing a quadric regression equation through response surface methodology accurately reflected the interrelation among the polysaccharides yield rate, liquid ratio, time and temperature .Experimental value was very close to the theoretically predicted value.This optimization of the extraction technology was good for application.