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中国精品科技期刊2020
介孔二氧化硅制备固定化菠萝蛋白酶的研究[J]. 食品工业科技, 2013, (13): 166-169. DOI: 10.13386/j.issn1002-0306.2013.13.051
引用本文: 介孔二氧化硅制备固定化菠萝蛋白酶的研究[J]. 食品工业科技, 2013, (13): 166-169. DOI: 10.13386/j.issn1002-0306.2013.13.051
Study on mesoporous silica immobilization of bromelain[J]. Science and Technology of Food Industry, 2013, (13): 166-169. DOI: 10.13386/j.issn1002-0306.2013.13.051
Citation: Study on mesoporous silica immobilization of bromelain[J]. Science and Technology of Food Industry, 2013, (13): 166-169. DOI: 10.13386/j.issn1002-0306.2013.13.051

介孔二氧化硅制备固定化菠萝蛋白酶的研究

Study on mesoporous silica immobilization of bromelain

  • 摘要: 采用经扫描电镜和透射电镜表征的介孔二氧化硅制备固定化菠萝蛋白酶,具有工艺简便、条件温和及操作方便的特点。考察了给酶量、pH和时间对固定化菠萝蛋白酶活力的影响,在酶量25mg/mL、pH5.0、固定化时间22h时,固定化酶的酶活为603U/g。介孔二氧化硅固定的菠萝蛋白酶在50℃水浴60min后,固定化酶的活力保持在92%以上,说明固定化酶的热稳定性较高。该固定酶持续操作三次后,使用活性逐渐衰减,但第3次相对酶活仍然达38.5%,反映了菠萝蛋白酶分子与载体有较强的物理吸附作用而且固定的菠萝蛋白酶有一定的可重复使用性。 

     

    Abstract: Scanning electron microscopy and transmission electron microscopy were used to characterize mesoporous silica, which was applied in preparation of immobilized bromelain. There are a number of clear superiority with simple process, mild condition and convenient operation. The concentrations of enzyme, pH and reaction time on the immobilized bromelain activity were investigeted.When conditions of the reaction were enzyme concentration of 25mg/mL, pH5.0, fixed time 22h, immobilized enzyme activity was 603U/g.Immobilized bromelain in water bath 50℃ for 60min, the enzyme activity remained more than 92% . The result indicated immobilized enzyme had higher thermal stability than esolvase. When the immobilized enzyme was continuously operated for three times, the relative enzyme activity still amounted to 38.5% for the third time.The conclusion showed that the bromelain molecules and vectors had a strong physical adsorption and the immobilized bromelain had certain reusability, though the activity was gradually declined.

     

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