款冬花硒多糖的制备及抗氧化性研究

Study on preparation and antioxidant activity of the Seleno-polysaccharides from Tussilago farfara

摘要: 本文对款冬花多糖（TFPs）进行硒化修饰工艺优化研究,并初步探讨了其抗氧化活性。研究采用亚硒酸钠硒化法,制备款冬花硒化多糖（STFPs）;以多糖中硒含量为指标,采用响应面法对制备工艺条件进行优化;并进行STFPs清除DPPH·、O-2·和OH·的实验,研究其体外抗氧化活性。得到最佳硒化条件为:硒化试剂质量比（m（亚硒酸钠）∶m（款冬花多糖））=1.06∶1,反应温度70℃,时间11h,硝酸体积分数0.5%。此时STFPs中的平均硒含量为3.84mg/g,与TFPs相比,清除DPPH自由基的能力显著提高,对O-2,OH·的清除能力也有一定程度的提高。

Abstract: To optimize the selenizing modification conditions of Tussilago farfara polysaccharides and its antioxidant activity were studied. Polysaccharides from Tussilago farfara was selenized as the sodium selenite method. According to the content of selenite, response surface methodology was operated to optimizing the selenizing modificiation.The effects of selenizing Tussilago farfara polysaccharides (STFPs) on scavenging DPPH was determined.The result showed that 1.06 ∶ 1 of fraction of selenite reagents, 70℃, 11h, 0.5% of volume fraction of HNO3 were the optimum conditions.The average content of selenium in STFPs was 3.84mg /g.Compared with TFPs, the effect of STFPs had been improved on scavenging DPPH, O-2, OH·and the most effect of DPPH·scavenging.