Abstract: β-glucosidase is widely found in nature, and plays an important role in citrus. In this paper, the methods of determining the activity of β-glucosidase, and the separation and purification of β-glucosidase were briefly introduced. The effect of β-glucosidase on the release of bound aroma compounds was introduced in detail, and the role on anthocyanin degradation and limonoid glucosides release were also summarized. In addition, the problems of the research on β-glucosidase in citrus and the research direction were also stated.