Abstract: The amphiphilic molecule was prepared by conjugating soy β-conglycinin and dextran (MW 67ku) under dry-heated reaction followed by trypin hydrolysis with the degree of hydrolysis (DH) at 2.2%. Nanoparticles were synthesized from β-conglycinin , β-conglycinin-dextran conjugate products and hydrolysates of β-conglycinin-dextran conjugate products DH 2.2% by desolvation method. The effect of glutaraldehyde cross-linking and removing ethanol by evaporation on the formed nanoparticles were examined. Formed nanoparticles were systematically investigated by means of particle size, zeta-potential and polydispersity index (PDI) . Self-assembly could be easily occurred in the hydrolysates of β-conglycinin-dextran conjugate products DH 2.2% during desolvation process, and the formed nanoparticles had smaller particle size, much higher absolute value of zeta-potential and a significantly lower PDI compared with that of the nanoparticles formed from β-conglycinin, and β-conglycinin-dextran conjugate products.