Abstract: The aim of this study was to develop whether the polysaccharides extracted from H.fusiformis have the anti-fatigue effect on mice. It was important for the further development of H. fusiformis. The weight, loading swimming time, the activities of blood SOD, the content of blood MDA, liver glycogen, muscle lactic acid, the content of blood urea nitrogen were measured after 15 days through being given different doses (50、150mg/mL·d) of the polysaccharides to mice. The result showed there was no significant difference on the body weights between control and polysaccharides fed mice (p>0.05) . However, the loading swim time (p<0.01) , the activity of SOD (p<0.01) and the concentration of liver glycogen (p<0.01) were enhanced, while the concentration of MDA (p<0.01) , muscle lactic acid (p<0.01、0.05) , and urea in blood (p<0.01) were decreased. In conclusion, the polysaccharides extracted from H. fusiformis do had anti-fatigue effect on mice.