Abstract: Employing the portamex, the antioxidative hydrolysates were successfully produced by the hydrolysis method under the condition of pH7.5, 50℃ and 3h. The obtained hydrolysates showed high antioxidative ability in vitro, which clearance rate of DPPH radical was 93.14%. The IC50 values of hydrolysates for superoxide anion and DPPH radical were 7.97mg/mL and 2.89mg/mL, respectively. According to the animal experiments, the results showed that the hydrolysates could decrease the content of MDA by 57.4% and increase the activities of CAT and SOD by 20.1% and 4.2%, respectively. As a result, the conclusion can be drawn that the hydrolysates obtained possessed high antioxidative ability both in vivo and in vitro.