Abstract: To investigate the growth inhibitory effect of Lavender essential oil on HepG2 cells. The growth inhibitory effect of Lavender essential oil on HepG2 was detected using MTT assay , the morphology of cells was observed with HE staining, and the apoptosis was assayed with the flow cytometry. Results:Lavender essential oil inhibited the growth of HepG2 cells at a concentrate-dependent manner. However, there was no significant difference between the inhibitory ratios of the 20% serum containing Lavender essential oil on HepG2 and that of 5-fluorouracil. The treatment with Lavender essential oil resulted in the reducion of cell number and volume, condensed cytoplasm and nuclei of cells, as well as apoptosis and necrosis of cells. Results showed that Lavender essential oil inhibits the growth of HepG2 cell via the apoptotic and necrotic manner.