Abstract: The subject prepared liposomes with soybean phosphatidylcholine (PC) and used the liposome as bilayer membrane structure model to understand the effect of oxidation on membrane.Fenton reaction was used to control the oxidation of liposome, and its properties including MDA, particle size, Zeta, appearance, membrane polarity, entrapment efficiency and membrane liquidity was measured.The degree of oxidation could be controlled by the reaction time of Fenton, with the Fenton reagent adding, the particle size of the liposomes first became larger and then smaller, and its distribution was more uneven.The ζ-potential, entrapment efficiency and membrane liquidity declined.In addition, the polarity of membrane increased slightly.