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中国精品科技期刊2020
免疫亲和柱净化-高效液相色谱法测定乳粉中游离叶酸含量[J]. 食品工业科技, 2013, (06): 88-90. DOI: 10.13386/j.issn1002-0306.2013.06.019
引用本文: 免疫亲和柱净化-高效液相色谱法测定乳粉中游离叶酸含量[J]. 食品工业科技, 2013, (06): 88-90. DOI: 10.13386/j.issn1002-0306.2013.06.019
Determination of free folic acid content in milk powder by immunoaffinity column purification-HPLC[J]. Science and Technology of Food Industry, 2013, (06): 88-90. DOI: 10.13386/j.issn1002-0306.2013.06.019
Citation: Determination of free folic acid content in milk powder by immunoaffinity column purification-HPLC[J]. Science and Technology of Food Industry, 2013, (06): 88-90. DOI: 10.13386/j.issn1002-0306.2013.06.019

免疫亲和柱净化-高效液相色谱法测定乳粉中游离叶酸含量

Determination of free folic acid content in milk powder by immunoaffinity column purification-HPLC

  • 摘要: 建立了免疫亲和柱净化-高效液相色谱法测定乳粉中游离叶酸的方法。该方法样品处理为样品用磷酸盐缓冲液溶解后,样液通过叶酸免疫亲和柱净化、甲醇洗脱液洗脱、浓缩富集后直接测定。色谱条件为:Waters C18柱(4.6mm×250mm,5μm),以20mg己烷磺酸钠+10mL冰乙酸+1.3mL三乙胺定容1L,pH3.2):甲醇(80∶20,V/V)作为流动相;流速为1mL/min;检测波长为280nm。标准曲线回归方程y=-185.68+290.29x,相关系数R2=0.9997;回收率为88.2%;RSD为1.99%。本方法检出限为40μg/kg。该法较国标方法具有样品处理简单方便、灵敏度高、重现性好、分析时间短等优点,可以满足调制乳粉及婴幼儿配方乳粉中叶酸含量的测定。 

     

    Abstract: To develop immunoaffinity column purification-high performance liquid chromatographic method for the determination of free folic acid in milk powder. Sample processing of the method for the sample dissolved with phosphate buffer, the sample liquid through folate immunoaffinity column purification, eluent of methanol, and concentrated to a direct determination after enrichment. These samples were separated on Waters C18 column (250mm×4.6mm, 5μm) , eluted with mixture of (20mg of sodium hexanesulfonate+10mL+1.3mL of glacial acetic acid and triethylamine the constant volume 1L, pH3.2) :methanol (80∶20, V/V) at 1mL/min, and detected at 280nm. Standard curve regression equationy=-185.68+290.29x, a correlation coefficient of R2=0.9997. Recovery was 88.2%, RSD was 1.99%. The method detection limit was 40μg/kg. Compared to the national standard method, the method had the advantage of easy sample handling, high sensitivity, good reproducibility and short analysis time to meet that modulation milk powder and infant formula milk powder folic acid content determination.

     

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