Abstract: Flavonoids was extracted from the chestnut male flowers with alcohol extracted method, microwave extracted method, ultrasonic wave and enzymatic extracted method, and the difference of total flavonoids was measured by different extraction method obtaining total reduction capacity, scavenging ability to DPPH and hydroxyl free radical by ultraviolet spectrophotometry.The results showed that total flavonoids by different extraction method obtaining had strong antioxidant activity.Total flavonoids by different extraction method obtaining scavenging ability to DPPH and hydroxyl free radical was alcohol extracted method >microwave extracted method>ultrasonic wave extracted method>enzymatic extracted method.The order of total reduction capacity was microwave extracted method > alcohol extracted method > ultrasonic wave extracted method > enzymatic extracted method.