斑点叉尾鮰鱼皮胶原蛋白的理化特征研究

Physical and chemical properties of collagen from the skin of channel catfish

摘要: 选用斑点叉尾鮰（Ictalurus punctatus）鱼皮为材料,用乙酸和乙酸-胃蛋白酶,分别提取鱼皮中的酸溶性胶原蛋白（acid-solubilise collagen,ASC）和酶溶性胶原蛋白（pepsin-solubilise collagen,PSC）,并对其理化性质特征进行研究。研究发现,提取得到的ASC纯度高达93.11%,PSC纯度高达93.46%;紫外吸收分析表明,ASC和PSC的吸收峰值均在233nm处;蛋白图谱中两种胶原蛋白均由两种不同的α链（α1）2α2组成,具备I型胶原蛋白的特征;ASC和PSC的傅里叶红外图谱相似,具有完整的三螺旋结构;ASC的变性温度为34.2℃,PSC的变性温度为33.9℃。

Abstract: The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from skin of channel catfish by the method of acid and acid-pepsin, and their physical and chemical characterization were analysed.The study indicated that the ASC had high purity of 93.11% and PSC of 93.46%, the analysis of UV spectra of ASC and PSC showed that the maximum absorption was obtained at 233nm.Both ASC and PSC consisted of two different α-chains (α 1) 2 α 2 were characterized as type I.Fourier transform infrared (FTIR) spectra of both ASC and PSC were almost similar with whole triple-helical structure.Transition temperature of ASC and PSC was 34.2℃ and 33.9℃.