Abstract: Polysaccharide of Acanthopanax obovatus Hoo was extracted with water and ethanol.Decoloration of Acanthopanax obovatus Hoo was studied with hydrogen peroxide solution method and the method of resin.With the single-factor and orthogonal test, the optimal technological conditions for Hydrogen peroxide solution method were determined as follows:temperature 60℃, time 3h, pH =10.0, sample liquid ratio 1:1.Under these conditions, the results were as follows:84.45% decoloration rate, 90.94% polysaccharides holding rate.While the optimal technological conditions for the method of resin were determined as follows:LS-700B, temperature 45℃, pH=7.0, experimental results were as follows:decoloration rate:79.50%, polysaccharides holding rate:82.80%, protein removal rate:79.10%.The results indicated hydrogen peroxide solution method was a relatively optimized decoloring method.