响应面分析优化酶法提取南瓜籽油的工艺研究

Optimization of emzyme-assisted extraction processing of oil from pumpkin seed by response surface methodology

摘要: 采用纤维素酶辅助提取南瓜籽油,在单因素实验的基础上,利用Box-Benhnken的中心组合设计原理及响应面分析法,建立用酶量、反应时间、反应温度与提取率之间的数学模式,确定酶法提取南瓜籽油的较佳工艺为用酶量17mg/g,酶解时间为2.64h,酶解温度为47℃。南瓜籽油提取率达89.12%。

Abstract: The cellulase-assisted extraction processing of oil from pumpkin seed was studied.On the basis of single -factor, the extraction conditions of oil from pumpkin seed by cellulase was optimized through Box-Benhnken design and response surface methodology.The mathematical regress model was established about extraction rate and enzyme dosage, time and temperature. According to this model, the optimum extraction conditions were determined as enzyme dosage 17mg /g, time 2.64h and temperature 47℃ .The extraction rate for this optimum condition was 89.12%.