Abstract: 79 batches of bee pollen that belonged to 15 varieties collected from 23 provinces all over the country during the year of 2008 ～ 2011 were tested for general content of flavones using optimized spectrophotometer. Results of the tests showed that the general content of flavones were in range of 0.49% ～ 3.49% . Among the 15 varieties, cole’s was the highest in terms of general content of flavones ( to be 3.15% in average) , rose’s and Schisandra’s were the second and the third high ( to be 2.20% and 2.04% , respectively, in average) , and sesame’ s was the one that was minimum in terms of general content of flavones ( to be 0.52% in average) . Statistic analysis indicated that general content of flavones differed among the provinces or /and the years in which the pollens were collected but the extent of difference was trivial within a given variety.Regardless of the variety and the year in which it was collected, the pollen reached its highest in terms of general content of flavones in Qinghai ( to be 2.91% in average) , its second and third high in Xinjiang and Guangdong ( to be 2.55% and 1.93% in average) , respectively, and its least in Heilongjiang and Hainan ( both to be 0.64% in average) .The difference was insignificant, however, from statistic point of view.