Abstract: To use the soak temperature , action time, ozone concentration , the sterilization rate as indexes , the sterilization effect of ozone to brown rice germination during the soaking process was studied. In the meantime, the sterilizing condition was optimized by using the response surface method (RSM) :soaking temperature was 30℃, action time was 3.7min, ozone concentration was 5.3mg/L. Under optimum conditions, the sterilization rate could be reached to 99.994%.