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中国精品科技期刊2020
李卓, 晏日安, 曾永青. 超声波强化酶法合成L-抗坏血酸癸酸酯及其抗氧化性研究[J]. 食品工业科技, 2013, (02): 204-209. DOI: 10.13386/j.issn1002-0306.2013.02.061
引用本文: 李卓, 晏日安, 曾永青. 超声波强化酶法合成L-抗坏血酸癸酸酯及其抗氧化性研究[J]. 食品工业科技, 2013, (02): 204-209. DOI: 10.13386/j.issn1002-0306.2013.02.061
Synthesis of lipase-catalyzed L-ascorbyl decanoate in ultrasonic field and determination of its antioxidant activity[J]. Science and Technology of Food Industry, 2013, (02): 204-209. DOI: 10.13386/j.issn1002-0306.2013.02.061
Citation: Synthesis of lipase-catalyzed L-ascorbyl decanoate in ultrasonic field and determination of its antioxidant activity[J]. Science and Technology of Food Industry, 2013, (02): 204-209. DOI: 10.13386/j.issn1002-0306.2013.02.061

超声波强化酶法合成L-抗坏血酸癸酸酯及其抗氧化性研究

Synthesis of lipase-catalyzed L-ascorbyl decanoate in ultrasonic field and determination of its antioxidant activity

  • 摘要: 研究了在超声波场中以固定化脂肪酶(Novozym 435)为催化剂,在叔丁醇中合成L-抗坏血酸癸酸酯。超声波可以显著加速底物的溶解,并使反应时间由48h缩短到4h,产率可达到78.48%,产物纯度为98.6%,超声波处理下固定化脂肪酶可重复利用的次数为4次。抗氧化实验结果表明在一定质量浓度范围内,L-抗坏血酸癸酸酯具有很强的还原力、清除羟自由基、DPPH自由基能力能力,在等质量浓度下与L-抗坏血酸棕榈酸酯相当,且前两个指标优于TBHQ;将L-抗坏血酸癸酸酯添加到油脂中,其抗氧化能力也较优,说明L-抗坏血酸癸酸酯是一种很有潜力的抗氧化剂。 

     

    Abstract: The esterification of decanoic acid with L-ascorbic acid in the presence of tert-butanol acid as the solvent and immobilized lipase (Novozym誖435) as the catalyst by means of ultrasonic irradiation to obtain L-ascorbyl decanoate (L-AD) was studied. By using ultrasound the dissolution rate of the reactants could be accelerated greatly, the reaction time of esterification could be reduced from 48 to 4h. The optimal actual yield was 78.48%.The purity of target product was above 98.6% after recrystallized. The immobilized lipase could be reused 4 times in ultrasonic field. In antioxidant activities of L-ascorbyl decanoate were studied by the items of hydroxyl radical system, system of DPPH·, and reducing power. Its antioxidant effect were further evaluated by adding into the soybean and sunflower oil. The result indicated that L -ascorbyl decanoate within the given concentration range in dose-effect relationship showed good antioxidant activities on the clearance of hydroxyl radical, superoxide anion free radical and the reducing power of DPPH·. Furthermore, antioxidant activities of L-ascorbyl decanoate were comparable with that of L-ascorbyl palmitate, and the first two indexes were higher than that of TBHQ. Thus, L-ascorbyl decanoate was demonstrated to be a potential food antioxidant.

     

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