Abstract: With the development of yak industry, there producing more and more viscera byproducts. In order to comprehensively utilized these viscera byproducts, extraction optimization of taurine extract from yak viscera was carried out using uniform design method, and the content of taurine was determined by ultraviolet-visible spectrophotometry. It was found that maximum yield of taurine was obtained when meal/solvent ratio, autolysis temperature, extractive temperature and extractive time were 1∶3 (m/v) , 55℃ , 75℃ , 60min. Furthermore , the maximum taurine contents was (7.02±0.50) mg/g in yak liver, while (5.59±0.25) mg/g in kidney, (4.46±0.17) mg/g in lung and (2.50±0.30) mg/g in heart. It was shown that yak viscera byproducts had abundant taurine for further processing.