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中国精品科技期刊2020
荞麦和商品苦荞茶中总黄酮的含量测定[J]. 食品工业科技, 2013, (02): 58-60. DOI: 10.13386/j.issn1002-0306.2013.02.035
引用本文: 荞麦和商品苦荞茶中总黄酮的含量测定[J]. 食品工业科技, 2013, (02): 58-60. DOI: 10.13386/j.issn1002-0306.2013.02.035
Determination of total flavonoids in buckwheat and buckwheat goods[J]. Science and Technology of Food Industry, 2013, (02): 58-60. DOI: 10.13386/j.issn1002-0306.2013.02.035
Citation: Determination of total flavonoids in buckwheat and buckwheat goods[J]. Science and Technology of Food Industry, 2013, (02): 58-60. DOI: 10.13386/j.issn1002-0306.2013.02.035

荞麦和商品苦荞茶中总黄酮的含量测定

Determination of total flavonoids in buckwheat and buckwheat goods

  • 摘要: 目的:测定荞麦不同栽培种、植株不同部位以及不同商品苦荞茶中的总黄酮含量。方法:采用UV法测定总黄酮含量;通过回流法提取,NaNO3-Al(NO3)3法显色测定样品吸光度,以芦丁为标准溶液计算求得总黄酮含量。结果:芦丁在0.0030060.1503mg/mL浓度范围内线性关系良好,R2=0.9999;平均回收率为97.9%,RSD为1.95%。结论:不同栽培种荞麦总黄酮含量测定中,苦荞的总黄酮含量在13.6721.56mg/g之间,甜荞总黄酮含量在5.797.23mg/g之间,苦荞总黄酮含量总体高于甜荞;荞麦植株不同部位中叶的总黄酮含量最高,最高达到68.30mg/g;商品苦荞茶中,所测商品总黄酮含量在6.9616.78mg/g之间,较苦荞种子中总黄酮含量有一定程度减少。该方法简便快捷,结果准确,重现性好,适用于荞麦和商品苦荞茶中总黄酮含量的测定。 

     

    Abstract: Objective:To determine the content of total flavones in the different kinds of buckwheat, different parts of buckwheat and buckwheat goods. Method:UV spectrophotometry for determination of total flavonoids was used. Using Na (NO 3 ) -Al (NO 3 ) 3 to detect the absorbance of samples and then count the total flavonoids. Results:The linear range of rutin was 0.003006~0.1503mg/mL (R 2 =0.9999) with average recovery of 97.9% (RSD 1.95% ) . Conclusion:In different cultivated species of buckwheat, the concentration of total flavonoids of Fagopyrum tataricum (L.) Gaerth was 13.67~21.56mg/g, and that of F. esculentum Moench was 5.79~7.23mg/g. In different parts of buckwheat, the concentration of total flavonoids of leaves was the highest, up to 68.30mg/g. For buckwheat tea, the concentration of total flavonoids was 6.96~16.78mg/g, but they were lower than that of buckwheat seeds. This method was simple, accurate and reproducible for determination of total flavonoids of buckwheat and buckwheat goods.

     

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