Abstract: For the rapid detection of the resveratrol in red wine, a new method was developed to determine the resveratrol in red wine by three-dimensional fluorescence spectrometry. The relatively complete fluorescence spectrum information of resveratrol was obtained, and character peaks of resveratrol in the luminescence spectrum were indentified. The excitation and emission wavelength was 290 and 380nm, respectively. The good linear relationship between the fluorescence intensity and the concentration of resveratrol was over the range was 0².0mg/L. The detection limitation was 2.57×10 -6 mg/L, recovery rates were 95.18%¹00.76%. As a simple, rapid and sensitive analysis method, it was suitable for the rapid determination and quality control of resveratrol in the red wines without the complex pretreatment.