Abstract: The keep-alive transport technology without water of yellow catfish in the ecological ice-temperature zone, the impact factors, and changes of biochemical indexes in fish muscle and blood were studied.The results showed that ecological ice-temperature zone of yellow catfish was-1 ~ 4℃.After two days temporary culture, the yellow catfish could survive for 24h with pure oxygen at 2℃, and the catfish could successfully revive when put them into water at the ambient temperature.Glycogen content and pH in muscle decreased, whereas lactic acid content increased during keep-alive process.Levels of creatinine, blood glucose, lactic acid in blood were significantly increased, level of albumin was significantly decreased and level of uric acid was significantly increased.No significant alternations were observed in the other biochemistry parameters before and after keep-alive experiment.