Abstract: Pickles’ brine was added in the homemade carrot juice. Change the initial pH of the medium and the incubation temperature to proliferate the pickles’ microbe. And analyzed the change of pH, total acid, amino nitrogen, total sugar and nitrite during the fermentation of the pickles with the different microbe incubated before, the effects of different proliferation conditions to the pickles could be compared and the optimum initial pH could be determined as well as the incubation temperature. The results showed that the carrot juice was used to proliferate the pickles’ microbe, the best initial pH was 5. 0 ~ 6. 0, the incubation temperature was 20 ~ 25℃.