Abstract: In order to find a quick and economical method for determining total content of anthocyanins from red wine, single pH method, pH-differential method and substraction method were used and the difference between the results of the three methods was compared. The results indicated that linear relation between absorbanceand concentration determined by that three methods were matched well, their related coefficient are 0. 9977, 0. 9988 and 0. 9984, and there were no significant differences between them (p<0. 05) . All of these methods could be used to determine total content of anthocyanins from red wine.