Abstract: The use of enzymatic hydrolysis method was investigated for extraction of polysaccharide from pure corn that was used as raw material. Influences of pH, temperature and time of enzymatic hydrolysis, enzyme concentration on the yield of polysaccharide from purple corn were studied on the basis of the type of enzymes and the proper combination were confirmed. Optimal extraction process parameters of polysaccharide from purple corn were determined after the data was optimized by response surface method. The results showed that by employing cellulase, pectinase and papain in single enzyme method, mixed enzyme method and step enzymatic method, the stepwise enzymatic method got the highest yield rate of polysaccharide from purple corn. The optimal extraction process parameters:pH5. 5, temperature of enzymatic hydrolysis was 60℃, time of enzymatic hydrolysis was 170min, and enzyme concentration was 0. 03mg/mL. In the optimized extraction conditions, the extraction rate of purple corn polysaccharide was 8. 42%, approximate degree was 99. 41% compared with the prediction value.