荷叶中荷叶碱及原花青素提取工艺优化

Process optimization for extraction of procyanidins and nuciferine from the lotus leaf

摘要: 目的:优选荷叶中荷叶碱、原花青素提取工艺。方法:采用正交实验设计,以原花青素、荷叶碱的提取率为指标,考察磷酸浓度、提取时间、料液比等因素对提取率的影响,优选最佳工艺参数。结果:以0.3%(V/V)磷酸为提取溶剂,料液比为1∶25(g/L),提取时间1.5h,提取2次,原花青素的提取率为89.2%,荷叶碱的提取率为69.9%。结论:采用酸水法提取荷叶中荷叶碱、原花青素,具有提取效率高、工艺简便、成本低等特点,适合于工业化大生产应用。

Abstract: Objective:To optimize the extracting process of procyanidins and nuciferine from the lotus leaf simultaneously. Method:Orthogonal design was used to optimize the extracting process of procyanidins and nuciferine with the transfer rate as the evaluation index. The effect of several important factors such as the pH of extracting solvent, the volume fraction of acid, the extraction time, the material-liquid ratio and the extract times of procyanidins and nuciferine were studied. Result:The optimal extraction process was as follows:0. 3% (v/v) of phosphate water solution;the material-liquid ratio was 1∶ 25;two times with 90min per time. The transfer rate of procyanidins was 89. 2%, while the transfer rate of nuciferine was 69. 9%. Conclusion:The optimum extraction processes were stable, economical, feasible and adapts for industrialized production.