Abstract: Objective:To study laxative effect of L-arabinose and sucrose mixture in constipation mice. Methods:The experimental mice were given Loperamide Hydrochloride to set up the constipation mice model. Small intestinal propelling test and defecation test in mice were applied to observe the small intestinal propelling rate, the time of the first dejection, the dejection amount and weights in six hours, the stool shape. Results:In comparing with the model group, the low dose, middle dose and hight dose of L-arabinose and sucrose mixture could shorten the time of the first dejection, improve intestinal peristalsis apparently, increase dejection amount and weights in six hours, stool-softening. The effect of L-arabinose and sucrose mixture group Ⅰ was the best. Conclusion:The L-arabinose and sucrose mixture had the laxative effect in constipation mice .