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中国精品科技期刊2020
产高活性木聚糖酶放线菌的筛选及其产酶条件的优化[J]. 食品工业科技, 2012, (21): 172-177. DOI: 10.13386/j.issn1002-0306.2012.21.034
引用本文: 产高活性木聚糖酶放线菌的筛选及其产酶条件的优化[J]. 食品工业科技, 2012, (21): 172-177. DOI: 10.13386/j.issn1002-0306.2012.21.034
Screening of high-yield xylanase-producing Actinomycete sp. and optimization of its fermentation conditions[J]. Science and Technology of Food Industry, 2012, (21): 172-177. DOI: 10.13386/j.issn1002-0306.2012.21.034
Citation: Screening of high-yield xylanase-producing Actinomycete sp. and optimization of its fermentation conditions[J]. Science and Technology of Food Industry, 2012, (21): 172-177. DOI: 10.13386/j.issn1002-0306.2012.21.034

产高活性木聚糖酶放线菌的筛选及其产酶条件的优化

Screening of high-yield xylanase-producing Actinomycete sp. and optimization of its fermentation conditions

  • 摘要: 本研究采用平板透明圈法自土样中筛选出产木聚糖酶放线菌52株,其中酶活100U/mL以上的有12株。对其中产木聚糖酶酶活较高且产酶稳定的菌株F4712(初始酶活349.4U/mL)进行了液体发酵条件优化。实验首先对培养基成分包括碳源(种类及添加量)、氮源(种类及添加量)及发酵条件:温度、发酵初始pH、摇床转速以及发酵时间等因素进行了考察。通过Box-Behnken响应面分析对发酵产酶条件做进一步优化,确定了摇瓶发酵的最优条件。结果表明,最佳发酵条件为玉米芯水不溶性木聚糖(2.8%),酵母浸膏(0.5%)及蛋白胨(1.0%)、45℃、pH6.2、130r/min以及6d。在此条件下酶活达693.4U/mL(较优化前提高了98.5%)。 

     

    Abstract: 52 actinomycetes were isolated from the soil samples of different districts of China based on plate screening method, and 12 strains was obtained with enzyme activity over 100U/mL. Actinomycete F4712 with high xylanase-producing (the initial enzyme activity was 349. 4U/mL) was selected for further research. The conditions including:carbon source, nitrogen source, initial pH value, rotation rate, temperature and cultivation time were investigated by single-factor experiment. Box-Behnken was utilized to the response surface analysis to determine the conditions further, which were insoluble corncob xylan 2. 8%, a combination of beef peptone 1. 0% and yeast extract 0. 5%, pH 6. 2, 130r/min, 45℃ and 6 days. The xylanase activity was 693. 4U/mL under the optimal conditions, which was improved by 98. 5% compared to the original activity.

     

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