Abstract: The importance of polyunsaturated fatty acids (PUFAs) , especially ω-6/ω-3 series, is scientifically recognized in human nutrition and disease prevention. With the emphasis on nutrition on the rise, how to purify PUFAs has already been a hot topic in various areas. Some physical or chemical methods are popular nowadays. However, research is ongoing to obtain the more natural and efficient ways with biological methods like enzymatic purification. This artide presented a review of the advances regarding the purification of ω-6/ω-3 PUFAs with enzymatic ways including enzymatic hydrolysis, selective enrichment, and some other methods with high expectation which could be connected with enzymatic method efficiently. The process of purification of AA, LA, GLA, DHA, EPA had been emphasized. Enzymatic method was a promising way for the purification of PUFAs. And in that sense, this review was expected to be of interest.