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中国精品科技期刊2020
紫甘薯花色苷制备及其抗猪传染性胃肠炎病毒(TGEV)活性的研究[J]. 食品工业科技, 2012, (21): 349-352. DOI: 10.13386/j.issn1002-0306.2012.21.015
引用本文: 紫甘薯花色苷制备及其抗猪传染性胃肠炎病毒(TGEV)活性的研究[J]. 食品工业科技, 2012, (21): 349-352. DOI: 10.13386/j.issn1002-0306.2012.21.015
Preparation and anti-TGEV activity of anthocyanin from purple sweet potato[J]. Science and Technology of Food Industry, 2012, (21): 349-352. DOI: 10.13386/j.issn1002-0306.2012.21.015
Citation: Preparation and anti-TGEV activity of anthocyanin from purple sweet potato[J]. Science and Technology of Food Industry, 2012, (21): 349-352. DOI: 10.13386/j.issn1002-0306.2012.21.015

紫甘薯花色苷制备及其抗猪传染性胃肠炎病毒(TGEV)活性的研究

Preparation and anti-TGEV activity of anthocyanin from purple sweet potato

  • 摘要: 为研究紫甘薯花色苷对猪传染性胃肠炎病毒(TGEV)的抑制作用,实验纯化了紫甘薯花色苷提取物,并采用液质联机的方法鉴定其结构组成;将制备的花色苷提取物作用于侵染TGEV的猪睾丸(ST)细胞,研究对TGEV诱导ST细胞的形态学变化和凋亡情况的影响,揭示花色苷对TGEV的预防和治疗效果。结果表明:紫甘薯花色苷提取物中主要含8种花色苷。花色苷质量浓度低于60μg/mL时,ST细胞生长良好且无细胞毒性;接种TGEV后,20μg/mL的花色苷就能抑制TGEV的增殖,且在安全浓度范围内抑制程度成剂量关系。在此体外实验中,花色苷对TGEV的预防效果比治疗效果更明显。结论:紫甘薯花色苷在20~60μg/mL质量浓度范围内对TGEV有很好的抗病毒功效。 

     

    Abstract: To study the inhibitory action of anthocyanins from purple sweet potato (APSP) to TGEV in vitro, the sample of anthocyanins extract was purified and its structure was identified by HPLC-MS. The prepared anthocyanins extract was acting on disseminated TGEV in swine testicle cell, then research its influence on the morphological changes as well as apoptosis of ST cell and reveal its prevention and treatment on TGEV. The result showed that the extract of purple sweet potato contains eight major anthocyanins. When mass concentration of APSP was less than 60μg/mL, ST cell grow well without the cell toxicity. Introduced TGEV to ST, 20μg/mL APSP could restrain the proliferation of TGEV after 48h and 72h cultivation, and inhibition effect was dose-response in the security concentration range. At the same time, it was obvious that there was better prevention effect than treatment effect in this study. In summary, APSP had very good antiviral efficacy for TGEV in 20~60μg/mL.

     

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